Serine protease activity in boar seminal vesicles and its immunological similarity to sperm acrosin
نویسندگان
چکیده
منابع مشابه
Boar acrosin. I. Purification and preliminary characterization of a proteinase from boar sperm acrosomes.
Acrosin is a proteolytic enzyme used by sperm to digest a path through the zona pellucida of the ovum. In ejaculated sperm it is inactivated by a proteinase inhibitor from seminal plasma that also inhibits trypsin. This inhibitor is removed or inactivated during the residence in the female reproductive tract as a part of the capacitation process. The boar acrosin-inhibitor complex was partially...
متن کاملSecretory activity of boar seminal vesicle glands.
This paper reviews the recent research on the physiological role of peptide and protein substances synthesized in boar seminal vesicle glands. Secretions from these glands are the major components of seminal plasma. Unique biochemical properties of seminal vesicle proteins, which determine their numerous functions in the reproductive processes, are discussed. The discovery of phosphotyrosine ac...
متن کاملBoar acrosin. II. Classification, inhibition, and specificity studies of a proteinase from sperm acrosomes.
Acrosin, a proteolytic enzyme located in the acrosome of sperm, exhibits amidase, esterase, and proteinase activity on synthetic and natural substrates containing arginyl and lysyl residues. Highly purified acrosin preparations from boar acrosomes have endopeptidase activity cleaving only the carboxyl bonds of arginine and lysine with a strong preference for arginine bonds. The Michaelis consta...
متن کاملNovel Flow Cytometry Analyses of Boar Sperm Viability: Can the Addition of Whole Sperm-Rich Fraction Seminal Plasma to Frozen-Thawed Boar Sperm Affect It?
Boar semen cryopreservation remains a challenge due to the extension of cold shock damage. Thus, many alternatives have emerged to improve the quality of frozen-thawed boar sperm. Although the use of seminal plasma arising from boar sperm-rich fraction (SP-SRF) has shown good efficacy; however, the majority of actual sperm evaluation techniques include a single or dual sperm parameter analysis,...
متن کاملBoar seminal plasma exosomes maintain sperm function by infiltrating into the sperm membrane
Seminal plasma ingredients are important for maintenance of sperm viability. This study focuses on the effect of boar seminal plasma exosomes on sperm function during long-term liquid storage. Boar seminal plasma exosomes had typical nano-structure morphology as measured by scanning electron microscopy (SEM) and molecular markers such as AWN, CD9 and CD63 by western blot analysis. The effect on...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1994
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.1000461